A new generation of microarrays, SpliceArray™ microarrays determine the expression of individual alternatively spliced transcripts. 

It is believed that over 70% of all human genes undergo alternative splicing, producing numerous gene products with different functions from a single locus.

SpliceArray™ microarrays covering the entire human, mouse and rat genomes are now available on the Affymetrix GeneChip® platform. Custom arrays are also available on both the Affymetrix and the Agilent platforms.

Different from standard gene profiling microarrays, SpliceArray™ microarrays are specifically designed to monitor each isoform of an RNA splicing event.  This is achieved through the combined use of exon body and exon-exon junction probes to measure the expression levels of both isoforms in an alternatively spliced event.  Diaxonhit’s SpliceArray™ microarrays also contain discovery probes: sets of exon-exon junction probes and exon-intron junction probes aimed at detecting possible, but as yet unidentified, variants.

The probe configuration used on SpliceArray™ microarrays is covered by US Patent No. 6,881,571 and European patent EP1,062,364 as well other patents granted and pending worldwide.

To find out how Diaxonhit applies SpliceArray™ results to identify new drugs and diagnostics, see our Therapeutic discovery process and Diagnostic discovery process.